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This pattern arises from liver–kidney yin-fluid depletion with internal deficiency-heat; it is characterized by soreness and weakness of the lower back and knees, dizziness and tinnitus, heat in the five centers (palms, soles, and chest), and tidal fever with night sweats. Induced by adding levothyroxine sodium to the diet, combined with tail-clamping provocation.
Combined with tail-clamping stress (5 min/day) for 21 days
This pattern arises from liver yin insufficiency with hyperactive liver yang; it is characterized by headache and dizziness, irritability, a flushed face with red eyes, and elevated blood pressure. Induced by adding crude aconite (Fuzi) powder to the diet.
Spontaneously hypertensive rats (SHR), 3 consecutive weeks
This pattern arises from deficient qi failing to propel the blood, resulting in sluggish circulation; it is characterized by fatigue and weakness, a dull complexion, and a purple-dark tongue. Induced by adding a high-fat diet, combined with exhaustive swimming.
High-fat diet for 8 weeks first, then exhaustive swimming for 16 days
This model arises from heart qi deficiency with the heart losing its nourishment; it is characterized by reduced cardiac function, decreased exercise tolerance, and dyspnea. Induced by rationed feeding of a high-salt diet (8% NaCl).
Dahl salt-sensitive rats, 20 g/rat/day for 20 weeks
Heart qi deficiency arises from insufficiency of heart qi and malnourishment of the heart-mind; it is characterized by palpitations with shortness of breath, fatigue and weakness, and aggravation after exertion. Induced by adding propranolol to the diet, combined with food restriction and forced swimming.
Food restriction + weight-loaded swimming (5% body weight) for 13 days, then propranolol for 4 days
This model arises from kidney qi deficiency with insecurity of the Chong and Ren vessels; it is characterized by early-pregnancy embryo loss. Induced by adding hydrocortisone to the diet, combined with mifepristone.
Hydrocortisone on gestational days 1–9; mifepristone on day 10
This pattern arises from kidney deficiency with essence depletion and collateral stasis; it is characterized by reduced sperm count and motility. Induced by adding Tripterygium wilfordii glycoside tablets (GTW) to the diet, combined with epinephrine injection and ice-water bathing.
Combined with subcutaneous epinephrine injection + ice-water bath
Kidney yang deficiency arises from insufficiency of kidney yang with endogenous deficiency-cold; it is characterized by aversion to cold with cold limbs, listlessness, and reduced activity. Induced by adding salt-processed Phellodendron bark powder to the diet, combined with cold-environment stimulation.
Combined with cold environment (4 °C, 1 h/day)
Kidney yang deficiency arises from kidney yang insufficiency with impaired qi transformation; it is characterized by aversion to cold with cold limbs, soreness and weakness of the lower back and knees, clear profuse urine, and edema. Induced by adding adenine to the diet.
20–30 consecutive days
Kidney yang deficiency arises from insufficiency of kidney yang and failure of its warming function; it is characterized by aversion to cold with cold limbs, soreness and weakness of the lower back and knees, listlessness, and reduced sexual function. Induced by adding hydrocortisone to the diet.
Mice: 10 consecutive days; rats: 7–10 days
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